Recombinant rat FGF1 alpha protein (ab129142)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
-
Product name
Recombinant rat FGF1 alpha protein
See all FGF1 alpha proteins and peptides -
Biological activity
Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is = 0.1 ng/ml, in the presence of 10 µg/ml heparin, corresponding to a specific activity of = 1 x 107 units/mg. -
Purity
> 95 % SDS-PAGE.
Purity: > 95% by SDS-PAGE and HPLC analysis. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Rat -
Sequence
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D -
Predicted molecular weight
16 kDa -
Amino acids
16 to 155
-
Preparation and Storage
-
Alternative names
- Acidic fibroblast growth factor
- aFGF
- Beta-endothelial cell growth factor
see all -
Function
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
