Recombinant mouse VEGF Receptor 2 protein (ab134453)
Key features and details
- Expression system: Insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, ELISA
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Product name
Recombinant mouse VEGF Receptor 2 protein
See all VEGF Receptor 2 proteins and peptides -
Biological activity
ab134453 activity was measured by its ability to bind to immobilized recombinant Human VEGF165 in a functional ELISA. -
Purity
> 95 % SDS-PAGE.
ab134453 was determined to be > 95% pure by SDS-PAGE and silver stain. Endotoxin level: -
Expression system
Insect cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MESKALLAVALWFCVETRAASVGLTGDFLHPPKLSTQKDILTILANTTLQ ITCRGQRDLDWLWPNAQRDSEERVLVTECGGGDSIFCKTLTIPRVVGNDT GAYKCSYRDVDIASTVYVYVRDYRSPFIASVSDQHGIVYITENKNKTVVI PCRGSISNLNVSLCARYPEKRFVPDGNRISWDSEIGFTLPSYMISYAGMV FCEAKINDETYQSIMYIVVVVGYRIYDVILSPPHEIELSAGEKLVLNCTA RTELNVGLDFTWHSPPSKSHHKKIVNRDVKPFPGTVAKMFLSTLTIESVT KSDQGEYTCVASSGRMIKRNRTFVRVHTKPFIAFGSGMKSLVEATVGSQV RIPVKYLSYPAPDIKWYRNGRPIESNYTMIVGDELTIMEVTERDAGNYTV ILTNPISMEKQSHMVSLVVNVPPQIGEKALISPMDSYQYGTMQTLTCTVY ANPPLHHIQWYWQLEEACSYRPGQTSPYACKEWRHVEDFQGGNKIEVTKN QYALIEGKNKTVSTLVIQAANVSALYKCEAINKAGRGERVISFHVIRGPE ITVQPAAQPTEQESVSLLCTADRNTFENLTWYKLGSQATSVHMGESLTPV CKNLDALWKLNGTMFSNSTNDILIVAFQNASLQDQGDYVCSAQDKKTKKR HCLVKQLIILGMEASLGDRIAMP -
Predicted molecular weight
105 kDa -
Amino acids
20 to 660 -
Additional sequence information
N-terminal sequence: ASVGLPGDFL
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.58% Sodium chloride, 0.49% MES
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionLyophilized ab134453 is soluble in water and most aqueous buffers. It should be reconstituted in water or PBS to a concentration of not lower than 100µg/ml.
Images
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SDS-PAGE - Recombinant mouse VEGF Receptor 2 protein (ab134453)SDS-PAGE analysis of recombinant mouse soluble VEGFR-1 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
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Functional Studies - Recombinant mouse VEGF Receptor 2 protein (ab134453)Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse endogenous sKDR and sFlk-1 HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess.
