Recombinant mouse TNF alpha protein (Animal Free) (ab217460)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
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Product name
Recombinant mouse TNF alpha protein (Animal Free)
See all TNF alpha proteins and peptides -
Biological activity
Determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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Purity
> 98 % SDS-PAGE.
assessed also by HPLC -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL -
Predicted molecular weight
17 kDa -
Amino acids
80 to 235 -
Additional sequence information
Soluble form of TNF alpha.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
