Recombinant mouse Flt3 ligand/Flt3L protein (Active) (ab270071)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
Preparation and Storage
- FL
- Flt 3 ligand
- Flt 3L
Images
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Functional Studies - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)
Fully biologically active determined by the dose dependent proliferation of AML-5 cells.
ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.
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SDS-PAGE - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)SDS-PAGE analysis of ab270071.
The protein is heavily glycosylated and therefore runs as several bands.
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Mass Spectrometry - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)Mass determination by ESI TOF.
Predicted mass is 18570.46 +/- 10 Da by ESI TOF. Observed mass is 18571 Da. Additional masses (glycoforms) observed at +365 (Hex(1)HexNac(1)), +203 (HexNac) & +569 (Hex(1)HexNAc(2)).
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HPLC - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)HPLC analysis of ab270071.
Additional peaks at 4.50 and 4.75 minutes correspond to multiple glycoforms.