Jurkat nuclear extract lysate (TPA + CI stimulated) (ab14850)
Overview
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Product name
Jurkat nuclear extract lysate (TPA + CI stimulated)
See all Jurkat lysates -
General notes
Cell line: Jurkat (Human T cell lymphoblast-like).
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.