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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Influenza

Recombinant influenza a Influenza A Hemagglutinin protein (Active) (ab174043)

Recombinant influenza a Influenza A Hemagglutinin protein (Active) (ab174043)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: ELISA, SDS-PAGE

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Description

  • Product name

    Recombinant influenza a Influenza A Hemagglutinin protein (Active)
    See all Influenza A Hemagglutinin proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab174043 at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    R4NN21
  • Protein length

    Protein fragment
  • Animal free

    No
  • Carrier free

    Yes
  • Nature

    Recombinant
    • Species

      Influenza A
    • Predicted molecular weight

      57 kDa
    • Amino acids

      19 to 523
    • Tags

      His tag C-Terminus
    • Additional sequence information

      It contains AA Asp 19 - Gly 338, Leu 341 - Asp 523.
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab174043 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      Lyophilized from 0.22 μm filtered solution.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 95% PBS, 5% Trehalose

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

Images

  • ELISA - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)
    ELISA - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)

    Immobilized ab174043 at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.

  • SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)
    SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)

    SDS-PAGE analysis of Influenza A Virus Hemagglutinin H7 in reducing conditions. Gel stained overnight with Coomassie Blue. DTT-reduced Protein migrates as 63-75 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab174043? Please let us know so that we can cite the reference in this datasheet.

    ab174043 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 95% PBS, 5% Trehalose

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

    Images

    • ELISA - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)
      ELISA - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)

      Immobilized ab174043 at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.

    • SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)
      SDS-PAGE - Recombinant Influenza A Hemagglutinin protein (BSA and azide free) (ab174043)

      SDS-PAGE analysis of Influenza A Virus Hemagglutinin H7 in reducing conditions. Gel stained overnight with Coomassie Blue. DTT-reduced Protein migrates as 63-75 kDa.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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