Recombinant influenza a Influenza A Hemagglutinin protein (Active) (ab174043)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
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Product name
Recombinant influenza a Influenza A Hemagglutinin protein (Active)
See all Influenza A Hemagglutinin proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized ab174043 at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoCarrier free
YesNature
Recombinant-
Species
Influenza A -
Predicted molecular weight
57 kDa -
Amino acids
19 to 523 -
Tags
His tag C-Terminus -
Additional sequence information
It contains AA Asp 19 - Gly 338, Leu 341 - Asp 523.
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
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Form
Lyophilized -
Additional notes
Lyophilized from 0.22 μm filtered solution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Immobilized ab174043 at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.
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SDS-PAGE analysis of Influenza A Virus Hemagglutinin H7 in reducing conditions. Gel stained overnight with Coomassie Blue. DTT-reduced Protein migrates as 63-75 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab174043 has not yet been referenced specifically in any publications.
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
-
Immobilized ab174043 at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.
-
SDS-PAGE analysis of Influenza A Virus Hemagglutinin H7 in reducing conditions. Gel stained overnight with Coomassie Blue. DTT-reduced Protein migrates as 63-75 kDa.