Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human WFIKKN2 protein (ab134608)

Key features and details

  • Expression system: CHO cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, HPLC, Functional Studies

Description

  • Product name

    Recombinant human WFIKKN2 protein
    See all WFIKKN2 proteins and peptides

  • Biological activity

    Determined by its ability to inhibit Human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 µg/ml in the presence of 5ng/ml of Human Myostatin (GDF-8).

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% by SDS-PAGE gel and HPLC analyses.

  • Endotoxin level

  • Expression system

    CHO cells

  • Accession

    Q8TEU8

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      LPPIRYSHAG ICPNDMNPNL WVDAQSTCRR ECETDQECET YEKCCPNVCG TKSCVAARYM DVKGKKGPVG MPKEATCDHF MCLQQGSECD IWDGQPVCKC KDRCEKEPSF TCASDGLTYY NRCYMDAEAC SKGITLAVVT CRYHFTWPNT SPPPPETTMH PTTASPETPE LDMAAPALLN NPVHQSVTMG ETVSFLCDVV GRPRPEITWE KQLEDRENVV MRPNHVRGNV VVTNIAQLVI YNAQLQDAGI YTCTARNVAG VLRADFPLSV VRGHQAAATS ESSPNGTAFP AAECLKPPDS EDCGEEQTRW HFDAQANNCL TFTFGHCHRN LNHFETYEAC MLACMSGPLA ACSLPALQGP CKAYAPRWAY NSQTGQCQSF VYGGCEGNGN NFESREACEE SCPFPRGNQR CRACKPRQKL VTSFCRSDFV ILGRVSELTE EPDSGRALVT VDEVLKDEKM GLKFLGQEPL EVTLLHVDWA CPCPNVTVSE MPLIIMGEVD GGMAMLRPDS FVGASSARRV RKLREVMHKK TCDVLKEFLG LH

    • Predicted molecular weight

      60 kDa

    • Amino acids

      35 to 576

    • Specifications

    Our Abpromise guarantee covers the use of ab134608 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

      Functional Studies

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Constituent: 0.16% Sodium phosphate

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

    General Info

    • Alternative names

      • follistatin
      • GASP 1
      • GASP-1
      • Growth and differentiation factor-associated serum protein 1
      • hGASP 1
      • hGASP-1
      • immunoglobulin
      • kazal
      • kunitz and NTR domain-containing protein 2
      • kunitz and NTR domain-containing-related protein
      • UNQ9235/PRO31996
      • WAP
      • WAP follistatin immunoglobulin kunitz and NTR domain containing-related protein
      • WAP kazal immunoglobulin kunitz and NTR domain containing protein 2
      • WFIKKN-related protein
      • Wfikkn2
      • WFKN2_HUMAN
      see all

    • Function

      Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin.

    • Tissue specificity

      Primarily expressed in ovary, testis and brain, but not in liver. In fetal tissues, it is primarily expressed in brain, skeletal muscle, thymus and kidney.

    • Sequence similarities

      Belongs to the WFIKKN family.
      Contains 2 BPTI/Kunitz inhibitor domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Kazal-like domain.
      Contains 1 NTR domain.
      Contains 1 WAP domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab134608? Please let us know so that we can cite the reference in this datasheet.

ab134608 has not yet been referenced specifically in any publications.

Preparation and Storage

  • follistatin
  • GASP 1
  • GASP-1

Images

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