Recombinant human Wee1 protein (Tagged) (ab271795)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Wee1 protein (Tagged)
See all Wee1 proteins and peptides -
Biological activity
Specific Activity: ≥42 pmol/min/μg
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Lys4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.
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Purity
>= 75 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DTEKSGKRE FDVRQTPQVN INPFTPDSLL LHSSGQCRRR KRTYWNDSCG EDMEASDYEL EDETRPAKRI TITESNMKSR YTTEFHELEK IGSGEFGSVF KCVKRLDGCI YAIKRSKKPL AGSVDEQNAL REVYAHAVLG QHSHVVRYFS AWAEDDHMLI QNEYCNGGSL ADAISENYRI MSYFKEAELK DLLLQVGRGL RYIHSMSLVH MDIKPSNIFI SRTSIPNAAS EEGDEDDWAS NKVMFKIGDL GHVTRISSPQ VEEGDSRFLA NEVLQENYTH LPKADIFALA LTVVCAAGAE PLPRNGDQWH EIRQGRLPRI PQVLSQEFTE LLKVMIHPDP ERRPSAMALV KHSVLLSASR KSAEQLRIEL NAEKFKNSLL QKELKKAQMA KAAAEERALF TDRMATRSTT QSNRTSRLIG KKMNRSVSLT IY -
Predicted molecular weight
75 kDa including tags -
Amino acids
215 to 646 -
Tags
GST tag N-Terminus -
Additional sequence information
N-terminal GST-Thrombin-Tag
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Preparation and Storage
- DKFZp686I18166
- EC 2.7.10.2
- FLJ16446
Images
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SDS-PAGE - Recombinant Human Wee1 protein (Tagged) (ab271795)
SDS-PAGE analysis of ab271795.
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Functional Studies - Recombinant Human Wee1 protein (Tagged) (ab271795)Specific activity of ab271795 was ≥42 pmol/min/μg.