Recombinant human VEGF 165A protein (Active) (ab259412)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
Preparation and Storage
- glioma-derived endothelial cell mitogen
- MGC70609
- MVCD1
Images
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Functional Studies - Recombinant human VEGFA protein (Active) (ab259412)
Fully biologically active determined by the dose dependent increase in proliferation of Human umbilical vein endothelial cells.
ED50 is ≤ 5.3 ng/mL, corresponding to a specific activity of 0.19 x 106 units/mg.
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SDS-PAGE - Recombinant human VEGFA protein (Active) (ab259412)SDS-PAGE analysis of ab259412.
Protein migrates higher than expected due to glycosylation.
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Mass Spectrometry - Recombinant human VEGFA protein (Active) (ab259412)Mass determination by ESI TOF.
Predicted mass is 19223.56 (+/- 10 Da by ESI-TOF). Mass at 19066.94 is due to loss of arginine on the C-terminus; peak at 19099.52 is a process related impurity.
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HPLC - Recombinant human VEGFA protein (Active) (ab259412)HPLC analysis of ab259412.