Recombinant human UBE1 protein (Active) (ab271808)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human UBE1 protein (Active)
See all UBE1 proteins and peptides -
Biological activity
Reaction was performed in 50 mM Tris pH 7.4, 5 mM MgCl2, 1 mM DTT, 5 µM ATP, 10 µM Ub, and 0.05% Tween. Reaction was initiated with the addition of UBE1 and was incubated at 30°C for 45 mins. The luminescence was read with the Kinase Glo Max reagent.
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Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
2 to 1058 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Alternative names
- A1S9
- A1S9 protein
- A1S9T
see all -
Relevance
Ubiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin activating enzyme exists as two isoforms: E1a (117 kDa), localized predominantly in the nucleus, and E1b (110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme. -
Cellular localization
Cytoplasmic and Nuclear
Images
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Functional Studies - Recombinant human UBE1 protein (Active) (ab271808)Specific activity of ab271808.
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SDS-PAGE - Recombinant human UBE1 protein (Active) (ab271808)SDS-PAGE analysis of 2 µg ab271808.
