Recombinant human TNFSF18/GITRL protein (Active) (ab256521)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 7.40
Constituents: PBS, 5% Trehalose
0.22 µm filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human TNFSF18/GITRL protein (Active) (ab256521)
Immobilized ab256521 at 2 μg/mL (100 μL/well) can bind human GITR, Fc Tag with a linear range of 0.1-4 ng/mL.
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Functional Studies - Recombinant human TNFSF18/GITRL protein (Active) (ab256521)Immobilized ab256521 at 2 μg/mL (100 μL/well) can bind human GITR, mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-8 ng/mL.
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SDS-PAGE - Recombinant human TNFSF18/GITRL protein (Active) (ab256521)Reducing SDS-PAGE - Recombinant human TNFSF18/GITRL protein (Active) (ab256521).
The gel was stained overnight with Coomassie Blue.
The protein has a calculated MW of 20.5 kDa. The protein migrates as 23 kDa and 26 kDa under reducing conditions (SDS-PAGE) due to glycosylation.