Recombinant human TNF Receptor I protein (Active) (ab224647)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human TNF Receptor I protein (Active) (ab224647)Immobilized Human TNF-alpha (Tag Free) low endotoxin (HPLC-verified) at 2 µg/mL (100 µL/well) can bind Human TNFR1 (His Tag) with a linear range of 3-25 ng/mL (QC tested).
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SDS-PAGE - Recombinant human TNF Receptor I protein (Active) (ab224647)Human TNFR1 (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. -
Functional Studies - Recombinant human TNF Receptor I protein (Active) (ab224647)Immobilized Human TNF-alpha, Tag Free at 2 μg/ml (100 μl/well) can bind ab224647 with a linear range of 2-16 ng/ml.
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SDS-PAGE - Recombinant human TNF Receptor I protein (Active) (ab224647)ab224647 analyzed by SDS-PAGE under reducing conditions and stained overnight with Coomassie Blue.
The protein migrates as 30-35 kDa under reducing conditions due to different glycosylation.
