Recombinant human TNF alpha protein (Active) (ab155699)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
-
Functional Studies - Recombinant human TNF alpha protein (ab155699)
ab155699 induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D.
The EC50 for this effect is 0.007-0.014 ng/ml (Routinely tested).
-
Functional Studies - Recombinant human TNF alpha protein (ab155699)Neutralization assay shows that the cytotoxicity effect of ab155699 was inhibited by increasing concentration of Adalimumab.
The concentration of ab155699 used is 1 ng/mL.
The IC50 is 29 ng/mL (Routinely tested).
-
ELISA - Recombinant human TNF alpha protein (ab155699)Immobilized ab155699 at 2 μg/mL (100 μL/well) binds Humira.
Linear range: 0.2-2 ng/mL (QC tested).
-
ELISA - Recombinant human TNF alpha protein (ab155699)Immobilized ab155699 at 2 μg/mL (100 μL/well) binds ab174046.
Linear range: 0.2-3 ng/mL (Routinely tested).
-
Functional Studies - Recombinant human TNF alpha protein (ab155699)Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bindab155699 with an affinity constant of 0.255 nM, as determined in a SPR assay (Biacore T200) (Routinely tested).
-
SDS-PAGE - Recombinant human TNF alpha protein (ab155699)Reduced ab155699 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%
The protein migrates as 17 kDa and 18 kDa under reducing conditions.
-
SEC-MALS - Recombinant human TNF alpha protein (Active) (ab155699)Purity of ab155699 was more than 95% in HP-SEC, and around 47-60 kDa verified by SEC-MALS.
