Recombinant human Thrombin protein (Active) (ab269042)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Thrombin protein (Active)
See all Thrombin proteins and peptides -
Biological activity
The specific activity of ab269042 was >2,000 NIH units/mg in a thrombin digestion assay using recombinant GST-HER2 protein as substrate at room temperature and enzyme:substrate mass ratio of 1:120 for 1 and 3 hours, respectively.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
37 kDa by SDS-PAGE -
Amino acids
364 to 622 -
Additional sequence information
Thrombin heavy chain
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Preparation and Storage
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Alternative names
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all -
Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
Images
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Functional Studies - (ab269042)
The specific activity of ab269042 was >2,000 NIH units/mg in a thrombin digestion assay using recombinant GST-HER2 protein as substrate at room temperature and enzyme:substrate mass ratio of 1:120 for 1 and 3 hours, respectively.
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SDS-PAGE - (ab269042)SDS-PAGE analysis of ab269042.