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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases

Recombinant human SHP2 (mutated T253M + Q257L) protein (Active) (ab268900)

Recombinant human SHP2 (mutated T253M + Q257L) protein (Active) (ab268900)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human SHP2 (mutated T253M + Q257L) protein (Active)
    See all SHP2 proteins and peptides
  • Biological activity

    The specific activity of ab268900 was  5056 nmol phosphate released /min/mg in a phosphatase assay using tyrosine phosphopeptide-2 substrate (DADE(pY)LIPQQG).

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q06124
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Molecular weight information

      SDS-PAGE molecular weight: ~98kDa.
    • Modifications

      mutated T253M + Q257L
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      GenBank: NM_002834

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.87% Sodium chloride, 0.31% Glutathione, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine), 0.42% MOPS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Functional Studies - Recombinant human SHP2 protein (Tagged) (ab268900)
    Functional Studies - Recombinant human SHP2 protein (Tagged) (ab268900)

    The specific activity of ab268900 was  5056 nmol phosphate released /min/mg in a phosphatase assay using tyrosine phosphopeptide-2 substrate (DADE(pY)LIPQQG).

  • SDS-PAGE - Recombinant human SHP2 protein (Tagged) (ab268900)
    SDS-PAGE - Recombinant human SHP2 protein (Tagged) (ab268900)

    SDS-PAGE analysis of ab268900.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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