Recombinant human SET7 protein (ab198126)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 62% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human SET7 protein
See all SET7 proteins and peptides -
Biological activity
50 μl reaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 μM S-adenosylmethionine, and 0-10 ng SET9) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then incubate 30 mins with HRP-labeled secondary antibody. Finally, add HRP chemiluminescent substrates and read luminescence.
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Purity
>= 62 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE ISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKT YLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDK YLKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFFFFDGSTLE GYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQY KDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFI DGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDP YESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDW ALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVH PRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAF QATQQK -
Molecular weight information
63 kDa by SDS-PAGE -
Amino acids
52 to 266 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_030648
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.48% Tris, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.49% Glutathione, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Active human SETD7 protein fragment (ab198126)
Activity of ab198126
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SDS-PAGE - Active human SETD7 protein fragment (ab198126)SDS-PAGE analysis of ab198126.