Recombinant Human SEMA5A protein (His tag) (ab276380)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human SEMA5A protein (His tag)
See all SEMA5A proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
MKGTCVIAWL FSSLGLWRLA HPEAQGTTQC QRTEHPVISY KEIGPWLREF RAKNAVDFSQ LTFDPGQKEL VVGARNYLFR LQLEDLSLIQ AVEWECDEAT KKACYSKGKS KEECQNYIRV LLVGGDRLFT CGTNAFTPVC TNRSLSNLTE IHDQISGMAR CPYSPQHNST ALLTAGGELY AATAMDFPGR DPAIYRSLGI LPPLRTAQYN SKWLNEPNFV SSYDIGNFTY FFFRENAVEH DCGKTVFSRA ARVCKNDIGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE LQSTFFLPEL DLIYGIFTTN VNSIAASAVC VFNLSAIAQA FSGPFKYQEN SRSAWLPYPN PNPHFQCGTV DQGLYVNLTE RNLQDAQKFI LMHEVVQPVT TVPSFMEDNS RFSHVAVDVV QGREALVHII YLATDYGTIK KVRVPLNQTS SSCLLEEIEL FPERRREPIR SLQILHSQSV LFVGLREHVV KIPLKRCQFY RTRSTCIGAQ DPYCGWDVVM KKCTSLEESL SMTQWEQSIS ACPTRNLTVD GHFGVWSPWT PCTHTDGSAV GSCLCRTRSC DSPAPQCGGW QCEGPGMEIA NCSRNGGWTP WTSWSPCSTT CGIGFQVRQR SCSNPTPRHG GRVCVGQNRE ERYCNEHLLC PPHMFWTGWG PWERCTAQCG GGIQARRRIC ENGPDCAGCN VEYQSCNTNP CPELKKTTPW TPWTPVNISD NGGHYEQRFR YTCKARLADP NLLEVGRQRI EMRYCSSDGT SGCSTDGLSG DFLRAGRYSA HTVNGAWSAW TSWSQCSRDC SRGIRNRKRV CNNPEPKYGG MPCLGPSLEY QECNILPCPV DGVWSCWSPW TKCSATCGGG HYMRTRSCSN PAPAYGGDIC LGLHTEEALC NTQPCPESWS EWSDWSECEA SGVQVRARQC ILLFPMGSQC SGNTTESRPC VFDSNFIPEV SVARSSSVEE KRCGEFNM -
Predicted molecular weight
107 kDa including tags -
Molecular weight information
The recombinant human SEMA5A consists of 957 amino acids and predicts a molecular mass of 107 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA5A is approximately 130-140 kDa due to glycosylation. -
Amino acids
1 to 968 -
Tags
His tag C-Terminus -
Additional sequence information
Predicted N-terminus: Glu 23.
Specifications
Our Abpromise guarantee covers the use of ab276380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- SEMA5A
- SEMAF
- Semaphorin F
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Relevance
Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met. -
Cellular localization
Membrane; single-pass type I membrane protein
Images
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SDS-PAGE - Recombinant Human SEMA5A protein (ab276380)SDS-PAGE analysis of ab276380
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276380 has not yet been referenced specifically in any publications.
Preparation and Storage
- SEMA5A
- SEMAF
- Semaphorin F
Images
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SDS-PAGE - Recombinant Human SEMA5A protein (ab276380)SDS-PAGE analysis of ab276380