Recombinant human RNF20 protein (ab198645)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human RNF20 protein -
Biological activity
Assay Conditions: 50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin and ab198645 in 50 mM Tris (pH 7.4) incubate for 30 min at 37°C. Then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against RNF20, incubate 1 hour. Finally, incubate 1 hour with the HRP-labeled secondary antibody.
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Purity
>= 50 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTL QTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFD ENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEG QEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVEL LSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFS KLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNS KGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQ DFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAH LDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKE YEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKL REAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASK ASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESE KERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYR SAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDAL RKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQ QLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQV VRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQL ELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDN VPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYD TRQRKCPKCNAAFGANDFHRIYIG -
Predicted molecular weight
115 kDa including tags -
Amino acids
2 to 975 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
NM_019592.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 80 ng/µl DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human RNF20 protein (ab198645)
Activity activity using ab198645.
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SDS-PAGE - Recombinant human RNF20 protein (ab198645)10% SDS-PAGE analysis of 0.1 µg ab198645 with Coomassie staining.