Recombinant Human PPP2CB protein (ab128562)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
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Product name
Recombinant Human PPP2CB protein -
Purity
> 75 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
59 kDa including tags -
Amino acids
1 to 309 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Images
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Western blot - Recombinant Human PPP2CB protein (ab128562)All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution
Lane 1 :Recombinant Human PPP2CB protein (ab128562)
Lane 2 : Recombinant human PP2A-alpha protein (ab128557)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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SDS-PAGE - Recombinant Human PPP2CB protein (ab128562)SDS-PAGE showing ab128562 at approximately 59 kDa