Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11786(B)] to PPP2CB
  • Suitable for: IP, ICC/IF, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PPP2CB antibody [EPR11786(B)]
    See all PPP2CB primary antibodies

  • Description

    Rabbit monoclonal [EPR11786(B)] to PPP2CB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human PPP2CB.
    Database link: P62714

  • Positive control

    • 293T, HeLa, Human fetal brain, Human fetal heart and NIH 3T3 lysates; 293T and NIH3T3 cells.

  • General notes

     

     

     This product was previously labelled as PP2A beta

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11786(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: PPP2CB knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A431 whole cell lysate (20 µg)

    ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. Ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Immunoprecipitation - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

    Detection of PPP2CB by Western Blot of Immunprecipitate.
    293T cell lysate immunoprecipitated using ab168371 at 1/10 dilution.
    For WB detection ab168371 at 1/10000 dilution was used.
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow Cytometry - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Flow Cytometry - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

    Flow Cytometric analysis of permeabilized NIH 3T3 cells labeling PPP2CB with ab168371 at 1/100  dilution (red) or a rabbit IgG (negative) (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

    Immunofluorescent analysis of 293T cells labeling PPP2CB with ab168371 at 1/50 dilution.

  • Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Western blot - Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    All lanes : Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilution

    Lane 1 : 293T lysate
    Lane 2 : HeLa lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 36 kDa

  • Anti-PPP2CB antibody [EPR11786(B)] (ab168371)
    Anti-PPP2CB antibody [EPR11786(B)] (ab168371)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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