Recombinant human PLK1 protein (Active) (ab271716)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 83% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PLK1 protein (Active)
See all PLK1 proteins and peptides -
Biological activity
Specific Activity: ≥14 pmol/min/µg.Assay Conditions: 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 1 mg/ml Casein substrate and 20 μM ATP. Reaction was performed at 30°C for 45 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent. -
Purity
> 83 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
68 kDa including tags -
Amino acids
2 to 603 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.7% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human PLK1 protein (Active) (ab271716)
Specific activity of ab271716 was ≥14 pmol/min/µg.
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SDS-PAGE - Recombinant human PLK1 protein (Active) (ab271716)SDS-PAGE analysis of 2 µg ab271716.