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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Recombinant human PLK1 protein (Active) (ab271716)

Recombinant human PLK1 protein (Active) (ab271716)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 83% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human PLK1 protein (Active)
    See all PLK1 proteins and peptides
  • Biological activity

    Specific Activity: ≥14 pmol/min/µg.
     
    Assay Conditions: 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 1 mg/ml Casein substrate and 20 μM ATP. Reaction was performed at 30°C for 45 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent.
  • Purity

    > 83 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P53350
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      68 kDa including tags
    • Amino acids

      2 to 603
    • Tags

      His tag C-Terminus

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.7% Imidazole
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Functional Studies - Recombinant human PLK1 protein (Active) (ab271716)
    Functional Studies - Recombinant human PLK1 protein (Active) (ab271716)

    Specific activity of ab271716 was ≥14 pmol/min/µg.

  • SDS-PAGE - Recombinant human PLK1 protein (Active) (ab271716)
    SDS-PAGE - Recombinant human PLK1 protein (Active) (ab271716)

    SDS-PAGE analysis of 2 µg ab271716.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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