Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
Key features and details
- Expression system: Baculovirus
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
-
Product name
Recombinant human Peroxiredoxin 1/PAG protein
See all Peroxiredoxin 1/PAG proteins and peptides -
Biological activity
Specific Activity between 800 and 1000 pmol/min/µg. Please enquire as to activity of specific lots. 1.88 µg of Peroxiredoxin 1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min. -
Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
SDS-PAGE - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)Lane 1: ab79945 on 14% SDS-PAGE, Coomassie staining, 20µg.
Lane 2: Protein marker. -
Functional Studies - Recombinant human Peroxiredoxin 1/PAG protein (ab79945) -
Western blot - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.
