Recombinant human PCSK9 (mutated R194A) protein (Active) (ab223015)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
Images
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ELISA - Recombinant human PCSK9 (mutated R194A) protein (Active) (ab223015)
Immobilized Human LDL R, His Tag at 10 μg/mL (100 μL/well) can bind Recombinant human PCSK9 protein (ab223015) with a linear range of 0.078-1.25 μg/mL.
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SDS-PAGE - Recombinant human PCSK9 (mutated R194A) protein (Active) (ab223015)SDS-PAGE of reduced ab223015 stained overnight with Coomassie Blue. The protein migrates as 17 kDa and 66 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.
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Functional Studies - Recombinant human PCSK9 (mutated R194A) protein (Active) (ab223015)Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant human PCSK9 protein (ab223015) with an affinity constant of 3.33 nM as determined in BLI assay.
