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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins

Recombinant Human PCDH10 protein (ab153775)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

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Description

  • Product name

    Recombinant Human PCDH10 protein
  • Purity

    > 95 % SDS-PAGE.
    ab153775 is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. I is lyophilized from an 0.2 µM filtered solution.
  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    Q9P2E7
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      QLHYTVQEEQEHGTFVGNIAEDLGLDITKLSARGFQTVPNSRTPYLDLNL ETGVLYVNEKIDREQICKQSPSCVLHLEVFLENPLELFQVEIEVLDINDN PPSFPEPDLTVEISESATPGTRFPLESAFDPDVGTNSLRDYEITPNSYFS LDVQTQGDGNRFAELVLEKPLDREQQAVHRYVLTAVDGGGGGGVGEGGGG GGGAGLPPQQQRTGTALLTIRVLDSNDNVPAFDQPVYTVSLPENSPPGTL VIQLNATDPDEGQNGEVVYSFSSHISPRARELFGLSPRTGRLEVSGELDY EESPVYQVYVQAKDLGPNAVPAHCKVLVRVLDANDNAPEISFSTVKEAVS EGAAPGTVVALFSVTDRDSEENGQVQCELLGDVPFRPKSSFKNYYTIVTE APLDREAGDSYTLTVVARDRGEPALSTSKSIQVQVSDVNDNAPRFSQPVY DVYVTENNVPGAYIYAVSATDRDEGANAQLAYSILECQIQGMSVFTYVSI NSENGYLYALRSFDYEQLKDFSFQVEARDAGSPQALAGNATVNILIVDQN DNAPAIVAPLPGRNGTPAREVLPRSAEPGYLLTRVAAVDADDGENARLTY SIVRGNEMNLFRMDWRTGELRTARRVPAKRDPQRPYELVIEVRDHGQPPL SSTATLVVQLVDGAVEPQGGGGSGGGGSGEHQRPSRSGGGETSLDLTVDH HHHHH
    • Predicted molecular weight

      76 kDa including tags
    • Amino acids

      19 to 715
    • Tags

      His tag C-Terminus
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab153775 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      pH: 7.40
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
      It is not recommended to reconstitute to a concentration less than 100 µg/ml.
      Dissolve the lyophilized protein in 1X PBS.
      Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    General Info

    • Alternative names

      • DKFZp761O2023
      • KIAA1400
      • MGC133344
      • OL PCDH
      • Ortholog of OL pcdh
      • OTTHUMP00000220001
      • PCD10_HUMAN
      • PCDH10
      • PCDH19
      • Protocadherin 10
      • Protocadherin-10
      see all
    • Function

      Potential calcium-dependent cell-adhesion protein.
    • Tissue specificity

      Moderately expressed in all regions of the brain examined, as well as in testis and ovary, and low expression in all other tissues tested.
    • Sequence similarities

      Contains 6 cadherin domains.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q9P2E7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab153775? Please let us know so that we can cite the reference in this datasheet.

    ab153775 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      pH: 7.40
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
      It is not recommended to reconstitute to a concentration less than 100 µg/ml.
      Dissolve the lyophilized protein in 1X PBS.
      Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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