Recombinant human PARP7 protein (Tagged) (ab271651)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 33% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PARP7 protein (Tagged) -
Biological activity
Specific Activity: >40 pmol/min/μg
Assay Conditions: Assay was done according to PARP7 assay kit protocol. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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Purity
>= 33 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
KRRPLFRSCF ILLPYLQTLG GVPTQAPPPL EATSSSQIIC PDGVTSANFY PETWVYMHPS QDFIQVPVSA EDKSYRIIYN LFHKTVPEFK YRILQILRVQ NQFLWEKYKR KKEYMNRKMF GRDRIINERH LFHGTSQDVV DGICKHNFDP RVCGKHATMF GQGSYFAKKA SYSHNFSKKS SKGVHFMFLA KVLTGRYTMG SHGMRRPPPV NPGSVTSDLY DSCVDNFFEP QIFVIFNDDQ SYPYFVIQYE EVSNTVSI -
Predicted molecular weight
31 kDa including tags -
Amino acids
400 to 657 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
- ADP-ribosyltransferase diphtheria toxin-like 14
- ARTD14
- AW558171
Images
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SDS-PAGE - Recombinant Human PARP7 protein (Tagged) (ab271651)
SDS-PAGE analysis of ab271651.
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Functional Studies - Recombinant Human PARP7 protein (Tagged) (ab271651)Specific activity of ab271651 was >40 pmol/min/µg.