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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes ADP-ribosylation

Recombinant human PARP7 protein (Tagged) (ab271651)

Price and availability

435 552 ₸

Availability

Order now and get it on Thursday February 25, 2021

Recombinant human PARP7 protein (Tagged) (ab271651)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 33% SDS-PAGE
  • Active: Yes
  • Tags: DDDDK tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human PARP7 protein (Tagged)
  • Biological activity

    Specific Activity: >40 pmol/min/μg

    Assay Conditions: Assay was done according to PARP7 assay kit protocol. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

  • Purity

    >= 33 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q7Z3E1
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      KRRPLFRSCF ILLPYLQTLG GVPTQAPPPL EATSSSQIIC PDGVTSANFY PETWVYMHPS QDFIQVPVSA EDKSYRIIYN LFHKTVPEFK YRILQILRVQ NQFLWEKYKR KKEYMNRKMF GRDRIINERH LFHGTSQDVV DGICKHNFDP RVCGKHATMF GQGSYFAKKA SYSHNFSKKS SKGVHFMFLA KVLTGRYTMG SHGMRRPPPV NPGSVTSDLY DSCVDNFFEP QIFVIFNDDQ SYPYFVIQYE EVSNTVSI
    • Predicted molecular weight

      31 kDa including tags
    • Amino acids

      400 to 657
    • Tags

      DDDDK tag N-Terminus

Preparation and Storage

  • ADP-ribosyltransferase diphtheria toxin-like 14
  • ARTD14
  • AW558171

Images

  • SDS-PAGE - Recombinant Human PARP7 protein (Tagged) (ab271651)
    SDS-PAGE - Recombinant Human PARP7 protein (Tagged) (ab271651)

    SDS-PAGE analysis of ab271651.

  • Functional Studies - Recombinant Human PARP7 protein (Tagged) (ab271651)
    Functional Studies - Recombinant Human PARP7 protein (Tagged) (ab271651)

    Specific activity of ab271651 was >40 pmol/min/µg.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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