Recombinant human PARP14 protein (ab198628)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PARP14 protein -
Biological activity
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Enzyme reaction is conducted using a PARP14 Assay Kit at room temperature for 1 hour.
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Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE ISMLEGAVLDIRYGVSRIAYSKDFETLVDFLSKLPEMLKMFKDRLCHKTY LNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKY LKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFKEYQELNELQ KKLNNISLDHKRPLIKVLGISRDVMQARDEIEAMIKRVRLAKEQESRADC ISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNL NTYTATDTKGHSLSVQRLTKSKVDIPAHWSDKQQNFCVVELLPSDPEYNT VASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQLF HGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPD ANGRKHVYVRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSL FVAFYDYQAYPEYLITFRK -
Predicted molecular weight
67 kDa including tags -
Amino acids
1470 to 1801 -
Tags
His tag N-Terminus , GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.28% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human PARP14 protein (ab198628)
Specific activity of ab198628.
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SDS-PAGE - Recombinant human PARP14 protein (ab198628)4-20 % SDS-PAGE analysis of ab198628 with Coomassie staining.
Lane 1: 1 µg ab198628
Lane 2: Protein marker
