Recombinant Human Neutrophil Bactericidal Protein BP30 (ab192292)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Reconstitute for long term storage.
pH: 7.4
-
ReconstitutionLyophilized protein should be stored at
General Info
-
Alternative names
- Bactericidal permeability-increasing protein
- Bactericidal/permeability increasing protein
- BPI
see all -
Function
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. -
Tissue specificity
Restricted to cells of the myeloid series. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Domain
The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N-and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested. -
Cellular localization
Secreted. Cytoplasmic granule membrane. Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab192292 has not yet been referenced specifically in any publications.
-