Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human NECAP2 protein (ab185838)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus, His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human NECAP2 protein
    See all NECAP2 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab185838 is 0.2 µM filtered.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q9NVZ3

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMEESGYESVLCVKPDVHVYRIPPRATNRGY RAAEWQLDQPSWSGRLRITAKGQMAYIKLEDRTSGELFAQAPVDQFPGTA VESVTDSSRYFVIRIEDGNGRRAFIGIGFGDRGDAFDFNVALQDHFKWVK QQCEFAKQAQNPDQGPKLDLGFKEGQTIKLNIANMKKKEGAAGNPRVRPA STGGLSLLPPPPGGKTSTLIPPPGEQLAVGGSLVQPAVAPSSGGAPVPWP QPNPATADIWGDFTKSTGSTSSQTQPGTGWVQFLEHHHHHH

    • Predicted molecular weight

      32 kDa including tags

    • Amino acids

      1 to 263

    • Tags

      His tag C-Terminus , His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab185838 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

      Supplied as a 0.2 µM filtered solution

    General Info

    • Alternative names

      • Adaptin ear binding coat associated protein 2
      • Adaptin ear-binding coat-associated protein 2
      • FLJ10420
      • NECAP endocytosis associated 2
      • NECAP endocytosis associated protein 2
      • NECAP endocytosis-associated protein 2
      • NECAP-2
      • NECAP2
      • NECP2_HUMAN
      see all

    • Function

      Involved in endocytosis.

    • Sequence similarities

      Belongs to the NECAP family.

    • Domain

      The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.

    • Cellular localization

      Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab185838? Please let us know so that we can cite the reference in this datasheet.

ab185838 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    Supplied as a 0.2 µM filtered solution

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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