Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (ab135021)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein -
Biological activity
50 µl reaction mix (50 mM Tris, pH 8.8, 5 mM MgCl2, 4 M DTT, 20 µM Sadenosylmethionine, and 5-50 ng MLL complex) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, incubate 30 minutes with the secondary HRP labeled antibody. Finally, add HRP chemiluminescent substrate and read luminescence. -
Purity
>= 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MLL
FRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYN PNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRG LFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEV VDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTY DYKFPIEDASNKLPCNCGAKKCRKFLN
WDR5
ATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSV KFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNL LVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDE SVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTA SGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKT YTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHT DVVISTACHPTENIIASAALENDKTIKLWKSDC
ASH2L Isoform 2
DTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNY SFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSK DKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPG SKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSG KGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRY ILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLK ISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQP LGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLP EDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIF YKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRP MSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP
RBBP5
NLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIV IWDFLTRGIAKIISAHIHPVCSLCWSRDGHKLVSASTDNIVSQWDVLSGD CDQRFRFPSPILKVQYHPRDQNKVLVCPMKSAPVMLTLSDSKHVVLPVDD DSDLNVVASFDRRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSNTT AIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLV NRTPWKKCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLL DVAWHPVRPIIASISSGVVSIWAQNQVENWSAFAPDFKELDENVEYEERE SEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKAL LYLPIAPEVEDPEENPYGPPPDAVQTSLMDEGASSEKKRQSSADGSQPPK KKPKTTNIELQGVPNDEVHPLLGVKGDGKSKKKQAGRPKGSKGKEKDSPF KPKLYKGDRGLPL EGSAKGKVQAELSQPLTAGGAISELL
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 1.7% Imidazole
Constituents: 0.00001% Zinc chloride, 0.29% Tris HCl, 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.03% TCEPThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (ab135021)Graph demonstrating the activity of ab135021
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SDS-PAGE - Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein (ab135021)4-20% SDS-PAGE analysis of ab135021 stained with Coomassie Blue.