Recombinant human Met (c-Met) protein (ab42612)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Inhibition Assay
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Product name
Recombinant human Met (c-Met) protein
See all Met (c-Met) proteins and peptides -
Biological activity
40 U/µg. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate and 4 µg/ml recombinant c-Met. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
74 kDa including tags -
Amino acids
956 to 1390 -
Tags
GST tag N-Terminus
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Preparation and Storage
- AUTS9
- c met
- D249
Images
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Functional Studies - Recombinant human Met (c-Met) protein (ab42612)
Specific Activity: 64pmol/min/ug. cMet was incubated with 2uM substrate (Tyr2 peptide) for 1h at 30°C in a buffer containing 50mM HEPES (ph7.5), 10mM MgCl2, 1mM EDTA, 0.01% BRIJ-35 and 200uM ATP.
Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. -
SDS-PAGE - Recombinant human Met (c-Met) protein (ab42612)10% SDS-PAGE Gel, Stained with Coomassie Blue, 4μg of ab42612 loaded onto Gel.
