Recombinant human MELK (mutated T460M) protein (ab179957)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human MELK (mutated T460M) protein
See all MELK proteins and peptides -
Biological activity
The specific activity of ab179957 was determined to be 120 nmol/min/mg.
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Purity
> 80 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSD LPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS QDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLID FGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLY VLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKK RISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQT MEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFTDI KSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTES NGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNK NQHKREILTMPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVISPE RRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSA -
Predicted molecular weight
88 kDa including tags -
Amino acids
1 to 550 -
Modifications
mutated T460M -
Tags
proprietary tag N-Terminus -
Additional sequence information
NM_014791
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.88% Sodium chloride, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human MELK (mutated T460M) protein (ab179957)
Sample Kinase Activity Plot.
The specific activity of ab179957 was determined to be 120 nmol/min/mg.
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SDS-PAGE - Recombinant human MELK (mutated T460M) protein (ab179957)SDS-PAGE analysis of ab179957.
