Recombinant human MDH2 protein (ab99238)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, Functional Studies
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Product name
Recombinant human MDH2 protein
See all MDH2 proteins and peptides -
Biological activity
Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°CActivity Assay
- Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid.
- Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer.
- Mix by inversion and equilibrate to 1°C and monitor at A340nm until the value is constant using a spectrophotometer.
- Record the increase at A340nm for approximately 2 minutes
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Purity
> 95 % SDS-PAGE.
ab99238 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMAKVAVLGASGGIGQPLSLLLKNSPLVSRL TLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVP RKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAE VFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGK TIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAY AGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNL GIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK -
Predicted molecular weight
35 kDa including tags -
Amino acids
25 to 338 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human MDH2 protein (ab99238)15% SDS-PAGE showing ab99238 at approximately 35.2kDa (3µg).
