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Recombinant Human Legumain protein (ab151680)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human Legumain protein
    See all Legumain proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab151680 is more than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    Q99538

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      PIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVV MMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLR GDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNE TIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYA CYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVM QYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRK LMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLS ERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPL HRIKLSMDHVCLGHYVDHHHHHH

    • Predicted molecular weight

      49 kDa including tags

    • Amino acids

      18 to 433

    • Tags

      His tag C-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab151680 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • AEP
      • Asparaginyl endopeptidase
      • cysteine 1
      • Cysteine protease 1
      • EC 3.4.22.34
      • Legumain
      • LGMN
      • LGMN_HUMAN
      • LGMN1
      • Protease
      • Protease cysteine 1
      • Protease cysteine 1 (legumain)
      • PRSC1
      see all

    • Function

      Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

    • Tissue specificity

      Ubiquitous. Particularly abundant in kidney, heart and placenta.

    • Sequence similarities

      Belongs to the peptidase C13 family.

    • Post-translational
      modifications

      Glycosylated.

    • Cellular localization

      Lysosome.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab151680? Please let us know so that we can cite the reference in this datasheet.

ab151680 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Images

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