Recombinant human KDM4C / GASC1 / JMJD2C protein (Tagged) (ab198094)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 82% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human KDM4C / GASC1 / JMJD2C protein (Tagged)
See all KDM4C / GASC1 / JMJD2C proteins and peptides -
Biological activity
Specific Activity: 0.17 pmole/min/μg.
Assay Conditions: 10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab198094 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection. -
Purity
>= 82 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
EVAEVESPLNPSCKIMTFRPSMEEFREFNKYLAYMESKGAHRAGLAKVIP PKEWKPRQCYDDIDNLLIPAPIQQMVTGQSGLFTQYNIQKKAMTVKEFRQ LANSGKYCTPRYLDYEDLERKYWKNLTFVAPIYGADINGSIYDEGVDEWN IARLNTVLDVVEEECGISIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINY LHFGEPKSWYAIPPEHGKRLERLAQGFFPSSSQGCDAFLRHKMTLISPSV LKKYGIPFDKITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATVRWIDY GKVAKLCTCRKDMVKISMDIFVRKFQPDRYQLWKQGKDIYTIDHTKPTPA STPEVKAWLQRRRKVRKASRS -
Predicted molecular weight
69 kDa including tags -
Amino acids
2 to 372 -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank Accession No. BC143571
-
Preparation and Storage
- bA146B14.1
- GASC 1 protein
- GASC-1 protein
Images
-
Functional Studies - Recombinant human KDM4C / GASC1 / JMJD2C protein (Tagged) (ab198094)
Activity assay using ab198094.
-
SDS-PAGE - Recombinant human KDM4C / GASC1 / JMJD2C protein (Tagged) (ab198094)4-20% SDS-PAGE analysis of 0.8 µg ab198094 with Coomassie staining.
