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Cancer Tumor immunology Cytokines Interferons

Recombinant human Interferon alpha 1 protein (ab200301)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 96% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies, HPLC

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Description

  • Product name

    Recombinant human Interferon alpha 1 protein
    See all Interferon alpha 1 proteins and peptides
  • Biological activity

    The specific activity as determined in a viral resistance assay was found to be no less than 1.0 x 108 IU/mg

  • Purity

    > 96 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    P01562
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      22 kDa
    • Additional sequence information

      Approximately 19 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids corresponding to IFNa-1b polymorphism (A-V at position 137)
  • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab200301 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

        HPLC

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        pH: 7.40
        Constituents: 95% PBS, 4% Mannitol, 1% Human serum albumin

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL

      General Info

      • Alternative names

        • IFL
        • IFN
        • IFN alpha
        • IFN alpha 1/13
        • IFN alpha 1b
        • IFN alphaD
        • IFN-alpha-1/13
        • IFNA@
        • IFNA1
        • IFNA1_HUMAN
        • IFNA13
        • Interferon alpha 1
        • Interferon alpha-1/13
        • Interferon alpha-D
        • Interferon alpha1
        • Interferon, alpha 13
        • LeIF D
        see all
      • Function

        Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
      • Sequence similarities

        Belongs to the alpha/beta interferon family.
      • Cellular localization

        Secreted.
      • Target information above from: UniProt accession P01562 The UniProt Consortium
        The Universal Protein Resource (UniProt) in 2010
        Nucleic Acids Res. 38:D142-D148 (2010) .

        Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab200301? Please let us know so that we can cite the reference in this datasheet.

    ab200301 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • IFL
      • IFN
      • IFN alpha
      • IFN alpha 1/13
      • IFN alpha 1b
      • IFN alphaD
      • IFN-alpha-1/13
      • IFNA@
      • IFNA1
      • IFNA1_HUMAN
      • IFNA13
      • Interferon alpha 1
      • Interferon alpha-1/13
      • Interferon alpha-D
      • Interferon alpha1
      • Interferon, alpha 13
      • LeIF D
      see all
    • Function

      Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
    • Sequence similarities

      Belongs to the alpha/beta interferon family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01562 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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