Recombinant human IL-7 protein (ab155728)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE - Recombinant human IL-7 protein (ab155728)Human IL-7 (Tag Free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human IL-7 protein (ab155728)Human IL-7, Tag Free (ab155728) stimulates proliferation of PHA-P-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is 0.108-0.829 ng/mL
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Functional Studies - Recombinant human IL-7 protein (ab155728)Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (ab174078) on CM5 chip via anti-mouse antibodies surface can bind Human IL-7, Tag Free (ab155728) with an affinity constant of 13.3 nM as determined in a SPR assa
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Functional Studies - Recombinant human IL-7 protein (ab155728)Captured Human IL-7 R alpha, Fc Tag (ab174078) on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-7, Tag Free (ab155728) with an affinity constant of 23.9 nM as determined in a SPR assay
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Functional Studies - Recombinant human IL-7 protein (ab155728)Immobilized Human IL-7, Tag Free (ab155728) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Fc Tag (ab174078) with a linear range of 1-16 ng/mL
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SDS-PAGE - Recombinant human IL-7 protein (ab155728)SDS-PAGE analysis of reduced ab155728 staining overnight with Coomassie Blue.
