Recombinant human IL-25 protein (ab174086)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 5% Trehalose, 95% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.
Images
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SDS-PAGE - Recombinant human IL-25 protein (ab174086)Human IL-17E (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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SDS-PAGE - Recombinant human IL-25 protein (ab174086)Reduced SDS-PAGE analysis of ab174086. Staining overnight with Coomassie Blue.
Calculated MW of 16.7 kDa. DTT-reduced protein migrates as 24 kDa, 27 kDa and 31 kDa due to different glycosylation.