Recombinant Human IFN gamma Receptor beta/AF-1 protein (Fc Chimera) (ab83988)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human IFN gamma Receptor beta/AF-1 protein (Fc Chimera)
See all IFN gamma Receptor beta/AF-1 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical sequence: SQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYRVQFKYTDSKWF TADIMSIGVNCTQITATECDFTAASPSAGFPMDFNVTLRLRAELGALH SAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTA FFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWN KSNIFRVGHLSNISCYETMADASTELQQGSSNTKVDKKVEPKSCDKTH TCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYK CKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Amino acids
28 to 247 -
Additional sequence information
DNA encoding the signal peptide and extracellular domain of human IFN-gamma R2 (aa 1-247) chain was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.
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Preparation and Storage
- AF 1
- AF-1
- AF1
Images
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SDS-PAGE - Recombinant Human IFN gamma Receptor beta/AF-1 protein (Fc Chimera) (ab83988)Lane 1 – ab83988; Lane 2 – ab83988 treated with PNGase F to remove potential N-linked glycans; Lane 3 – ab83988 treated with a glycosidase cocktail to remove potential N- and O-linked glycans; Lane 4 – MW markers. 10 μg of protein was loaded per lane; Gel was stained with Coomassie G250.
Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes. -
Functional Studies - Recombinant Human IFN gamma Receptor beta/AF-1 protein (Fc Chimera) (ab83988)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW of the protein.