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Epigenetics and Nuclear Signaling Histones HMGs

Recombinant human HMGB1 protein (Tagged-His Tag) (ab81876)

Recombinant human HMGB1 protein (Tagged-His Tag) (ab81876)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: GSA, SDS-PAGE

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Description

  • Product name

    Recombinant human HMGB1 protein (Tagged-His Tag)
    See all HMGB1 proteins and peptides
  • Biological activity

    1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.

     

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    P09429
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGETKKKFKD PNAPKRPPSA FFLFCSEYRP KIKGEHPGLS IGDVAKKLGE MWNNTAADDK QPYEKKAAKL KEKYEKDIAA YRAKGKPDAA KKGVVKAEKS KKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDE
    • Predicted molecular weight

      27 kDa including tags
    • Amino acids

      2 to 215
    • Tags

      His tag N-Terminus

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Functional Studies - Recombinant Human HMGB1 protein (Tagged-His Tag) (ab81876)
    Functional Studies - Recombinant Human HMGB1 protein (Tagged-His Tag) (ab81876)

    ab81876 induces TNFα Production in RAW Cells.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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