Recombinant human HMGB1 protein (ab167718)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 99% PBS
Normally Mannitol or Trehalose are added as protectants before lyophilization.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionIt is recommended to reconstitute the lyophilized protein in 100µl sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
-
SDS-PAGE - Recombinant Human HMGB1 protein (ab167718)
SDS-PAGE of reduced ab1677 stained with Coomassie Blue.
DTT-reduced Protein migrates as 28 kDa and 32 kDa due to different glycosylation. -
Functional Studies - Recombinant Human HMGB1 protein (ab167718)ab167718 stimulates production of TNF-α in Raw-246.7 cells. The EC50 for this effect is 0.7855-0.8342 μg/mL.
