Recombinant human Glutaminase protein (ab198065)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 74% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Glutaminase protein -
Biological activity
Specific Activity: ≥ 25 nmol/min/µg
Assay Conditions:
The reaction mixture contained 100 mM Tris, pH 8.6, 150 mM K2HPO4, 25 mM L-glutamine, 2 mM NAD+, 4 units of GDH, and various amounts of ab198065 in a volume of 200 μl. Reactions were initiated with the addition of ab198065, and the increase in NADH absorbance at 340 nm was continuously monitored for 7 minutes at 37°C. Molar extinction coefficient of NADH is 6,220 M-1cm-1. -
Purity
>= 74 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
M HHHHHH SPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLH PWWGGGGWP AEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAP GPKDGPGETDAF GNSEGKE LVASGENKIKQGLLPSLEDLLFYTIAE GQEKIPVHKFITALKSTGLRTSDP RLKECMDML RLTLQTTSDGVML DKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDE LYESAKKQS GGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPL KY AIAVNDL GTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAG AIVVTSLIKQGVNN AEKFDYV MQFLNKMAGNEYVGFSNATFQSERE SGDRNFAIGYYLKEKKCFPEGTDMVG ILDFYFQ LCSIEVTCESASV MAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYD FSGQFAFH VGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLC N FHNYDN LRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRR FALSAMDMEQRD YDSRTAL HVAAAEGHVEVVKFLLEACKVNPFPKD RWNNTPMDEALHFGHHDVFKILQE YQVQYTP QGDSDNGKENQTVHK NLDGLL -
Predicted molecular weight
73 kDa including tags -
Amino acids
17 to 669 -
Tags
His tag N-Terminus -
Additional sequence information
This product is for the mature full length protein from aa 17 to 669. The signal peptide is not included.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.04% TweenThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Glutaminase protein (ab198065)
Example of specific activity of ab198065
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SDS-PAGE - Recombinant human Glutaminase protein (ab198065)4-20% SDS-PAGE analysis of 4 µg ab198065 with Coomassie staining.