Recombinant human FGF6 protein (Animal Free) (ab256002)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at room temperature. Store at -20°C.
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
0.2 micron filtered.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile water to 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Images
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SDS-PAGE analysis of ab256002 (1 μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.
4-20% Tris-Glycine gel. Coomassie Blue staining.
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ab256002 induced proliferation of NR6R-3T3 cells with 1 μg heparin.