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Recombinant Human ESAM protein (ab151384)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

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Description

  • Product name

    Recombinant Human ESAM protein
    See all ESAM proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    Q96AP7
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      QLQLHLPANRLQAVEGGEVVLPAWYTLHGEVSSSQPWEVPFVMWFFKQKE KEDQVLSYINGVTTSKPGVSLVYSMPSRNLSLRLEGLQEKDSGPYSCSVN VQDKQGKSRGHSIKTLELNVLVPPAPPSCRLQGVPHVGANVTLSCQSPRS KPAVQYQWDRQLPSFQTFFAPALDVIRGSLSLTNLSSSMAGVYVCKAHNE VGTAQCNVTLEVSTGPGAVDHHHHHH
    • Predicted molecular weight

      25 kDa including tags
    • Amino acids

      30 to 247
    • Tags

      His tag C-Terminus
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-ESAM antibody (ab74777)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab151384 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      pH: 7.20
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

    • Reconstitution
      Dissolve the lyophilized protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Reconstituted protein solution can be stored at 4-7oC for 2-7 days. Aliquots of reconstituted samples are stable at oC for 3 months.

    General Info

    • Alternative names

      • 2310008D05Rik
      • Endothelial cell adhesion molecule
      • Endothelial cell selective adhesion molecule
      • Endothelial cell-selective adhesion molecule
      • Esam
      • ESAM_HUMAN
      • HUEL (C4orf1) interacting protein
      • LP4791 protein
      • W117m
      see all
    • Function

      Can mediate aggregation most likely through a homophilic molecular interaction.
    • Tissue specificity

      Highly expressed in endothelial cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell junction > adherens junction. Cell junction > tight junction. Cell membrane.
    • Target information above from: UniProt accession Q96AP7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151384? Please let us know so that we can cite the reference in this datasheet.

    ab151384 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      pH: 7.20
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

    • Reconstitution
      Dissolve the lyophilized protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Reconstituted protein solution can be stored at 4-7oC for 2-7 days. Aliquots of reconstituted samples are stable at oC for 3 months.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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