Recombinant human ENO1 protein (Active) (ab89248)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.012% Magnesium sulphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant Human ENO1 protein (ab89248)
3 ug of reduced ab89248 on 15% SDS-PAGE, stained by Coomassie Blue.
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Western blot - Recombinant Human ENO1 protein (ab89248)Anti-ENO1 antibody (ab85086) at 1/1000 dilution +Recombinant human ENO1 protein (Active) (ab89248) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 30 seconds
