Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) (ab271497)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) -
Biological activity
Assay Conditions: Various amounts of ab271497 were incubated in 10-ul reaction with ARV-771 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
DVFLMIRRHK TTIFTDAKES STVFELKRIV EGILKRPPDE QRLYKDDQLL DDGKTLGECG FTSQTARPQA PATVGLAFRA DDTFEALCIE PFSSPPELPD VMKPQDSGSS ANEQAVQ -
Predicted molecular weight
14 kDa including tags -
Amino acids
2 to 118 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank: NM_007108
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Amino Acid Sequence 2
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Species
Human -
Sequence
DGEEKTYGGC EGPDAMYVKL ISSDGHEFIV KREHALTSGT IKAMLSGPGQ FAENETNEVN FREIPSHVLS KVCMYFTYKV RYTNSSTEIP EFPIAPEIAL ELLMAANFLD C -
Predicted molecular weight
13 kDa including tags -
Amino acids
2 to 112 -
Tags
His tag N-Terminus -
Additional sequence information
GenBank: NM_005648
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Amino Acid Sequence 3
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Species
Human -
Sequence
PRRAENWDEA EVGAEEAGVE EYGPEEDGGE ESGAEESGPE ESGPEELGAE EEMEAGRPRP VLRSVNSREP SQVIFCNRSP RVVLPVWLNF DGEPQPYPTL PPGTGRRIHS YRGHLWLFRD AGTHDGLLVN QTELFVPSLN VDGQPIFANI TLPVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD -
Predicted molecular weight
25 kDa including tags -
Amino acids
2 to 212 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank: NM_000551
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Description
Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged)
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged) (ab271497)
ab271497-BRD3 interaction.
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SDS-PAGE - Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged) (ab271497)SDS-PAGE analysis of ab271497.