Recombinant Human ECE1 protein (His tag) (ab276334)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human ECE1 protein (His tag)
See all ECE1 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
QYQTRSPSVC LSEACVSVTS SILSSMDPTV DPCHDFFSYA CGGWIKANPV PDGHSRWGTF SNLWEHNQAI IKHLLENSTA SVSEAERKAQ VYYRACMNET RIEELRAKPL MELIERLGGW NITGPWAKDN FQDTLQVVTA HYRTSPFFSV YVSADSKNSN SNVIQVDQSG LGLPSRDYYL NKTENEKVLT GYLNYMVQLG KLLGGGDEEA IRPQMQQILD FETALANITI PQEKRRDEEL IYHKVTAAEL QTLAPAINWL PFLNTIFYPV EINESEPIVV YDKEYLEQIS TLINTTDRCL LNNYMIWNLV RKTSSFLDQR FQDADEKFME VMYGTKKTCL PRWKFCVSDT ENNLGFALGP MFVKATFAED SKSIATEIIL EIKKAFEESL STLKWMDEET RKSAKEKADA IYNMIGYPNF IMDPKELDKV FNDYTAVPDL YFENAMRFFN FSWRVTADQL RKAPNRDQWS MTPPMVNAYY SPTKNEIVFP AGILQAPFYT RSSPKALNFG GIGVVVGHEL THAFDDQGRE YDKDGNLRPW WKNSSVEAFK RQTECMVEQY SNYSVNGEPV NGRHTLGENI ADNGGLKAAY RAYQNWVKKN GAEHSLPTLG LTNNQLFFLG FAQVWCSVRT PESSHEGLIT DPHSPSRFRV IGSLSNSKEF SEHFRCPPGS PMNPPHKCEV W -
Predicted molecular weight
80 kDa including tags -
Molecular weight information
The recombinant human ECE1 comprises 698 amino acids and has a predicted molecular mass of 80 kDa. As a result of glycosylation, rh ECE1 migrates as an approximately 125 kDa band in SDS-PAGE under reducing conditions. -
Amino acids
90 to 770 -
Tags
His tag N-Terminus -
Additional sequence information
Predicted N-terminus: His.
Specifications
Our Abpromise guarantee covers the use of ab276334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- ECE
- ECE 1
- ECE-1
see all -
Relevance
Function: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family. -
Cellular localization
Cell Membrane. Single pass type II membrane protein.
Images
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SDS-PAGE - Recombinant Human ECE1 protein (ab276334)SDS-PAGE analysis of ab276334
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276334 has not yet been referenced specifically in any publications.
Preparation and Storage
- ECE
- ECE 1
- ECE-1
Images
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SDS-PAGE - Recombinant Human ECE1 protein (ab276334)SDS-PAGE analysis of ab276334