Human MICB ELISA Kit (ab267804)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 59.9 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MICB ELISA Kit
See all MICB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.8% Inter-assay Sample n Mean SD CV% Serum 3 2.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
59.9 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 112% - 117% Serum 100 100% - 100% Hep Plasma 90 89% - 90% EDTA Plasma 97 97% - 98% Cit plasma 97 96% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MICB ELISA Kit (ab267804) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MICB protein in cell culture supernatant, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MICB with 59.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MHC class I polypeptide-related sequence B (MICB) is a stress induced ligand for the KLRK1/NKG2D receptor that neither associate with β2-microglobins nor bind peptides. The MICB protein is encoded by the MICB gene located within the MHC locus and are commonly expressed on tumors of epithelial origin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MICB Capture Antibody 1 x 600µl 10X Human MICB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human MICB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. -
Tissue specificity
Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. -
Involvement in disease
Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=The MICB*004 allele is associated with rheumatoid arthritis.
Note=Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. -
Sequence similarities
Belongs to the MHC class I family. MIC subfamily.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsProteolytically cleaved and released from the cell surface of tumor cells. -
Cellular localization
Cell membrane. Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum. - Information by UniProt
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Alternative names
- MHC class I chain related protein B
- MHC class I chain related protein B2
- MHC class I like molecule PERB11.2 IMX
see all -
Database links
- Entrez Gene: 4277 Human
- Omim: 602436 Human
- SwissProt: Q29980 Human
- Unigene: 731446 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MICB standard curve in Sample Diluent NS.The MICB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MICB in human cell culture supernatant sample.The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curves and corrected for sample dilution. Undiluted sample is as follows: MCF-7 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 520.4 pg/mL in MCF-7 supernatant.
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Sandwich ELISA - Human MICB ELISA Kit (ab267804)To learn more about the advantages of recombinant antibodies see here.
