Recombinant Human DcR3 protein (Fc Chimera) (ab185255)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 10% Trehalose, 0.61% Tris, 0.75% Glycine, 0.06% Sodium chloride, 0.44% L-Arginine -
ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.
Images
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SDS-PAGE - Recombinant Human DcR3 protein (Fc Chimera) (ab185255)
SDS-PAGE analysis of ab185255 in reducing conditions.
Gel stained overnight with Coomassie Blue.
DTT-reduced protein migrates as 60-65 kDa due to glycosylation.
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Functional Studies - Recombinant Human DcR3 protein (Fc Chimera) (ab185255)Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag, ab185255 with a linear range of 2-31 ng/mL.
