Recombinant human Cyclin D3/CCND3 + Cdk4 protein (Active) (ab271459)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 73% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Cyclin D3/CCND3 + Cdk4 protein (Active) -
Biological activity
Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
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Purity
>= 73 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE -
Predicted molecular weight
64 kDa including tags -
Amino acids
1 to 303 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
Genbank: NM_000075
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Amino Acid Sequence 2
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Species
Human -
Sequence
ELLCCEGTRH APRAGPDPRL LGDQRVLQSL LRLEERYVPR ASYFQCVQRE IKPHMRKMLA YWMLEVCEEQ RCEEEVFPLA MNYLDRYLSC VPTRKAQLQL LGAVCMLLAS KLRETTPLTI EKLCIYTDHA VSPRQLRDWE VLVLGKLKWD LAAVIAHDFL AFILHRLSLP RDRQALVKKH AQTFLALCAT DYTFAMYPPS MIATGSIGAA VQGLGACSMS GDELTELLAG ITGTEVDCLR ACQEQIEAAL RESLREASQT SSSPAPKAPR GSSSQGPSQT STPTDVTAIH L -
Amino acids
2 to 292 -
Tags
GST tag N-Terminus -
Additional sequence information
Genbank: NM_001760
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Preparation and Storage
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Cellular localization
Cyclin D3/CCND3: Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. Cdk4: Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Images
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Functional Studies - Recombinant Human CDK4 + CCND3 protein (Tagged) (ab271459)
Functional analysis of ab271459.
Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
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SDS-PAGE - Recombinant Human CDK4 + CCND3 protein (Tagged) (ab271459)SDS-PAGE analysis of ab271459.