Recombinant human CTRP5 protein (ab109041)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human CTRP5 protein -
Biological activity
Activates AMPK signaling pathway in rat L6 myoblasts. -
Purity
> 90 % SDS-PAGE.
ab109041 was 0.2µm filtered -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Human -
Predicted molecular weight
26 kDa including tags -
Amino acids
16 to 243 -
Tags
His tag N-Terminus
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab109041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 8.20
Constituents: 0.869% Tris HCl, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in distilled water
General Info
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Alternative names
- C1q and tumor necrosis factor related protein 5
- C1QTNF5
- Complement C1q tumor necrosis factor related protein 5 precursor
- LORD
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Relevance
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP5 has been suggested to be involved in age-related macular degeneration. -
Cellular localization
Secreted
Images
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Functional Studies - Recombinant human CTRP5 protein (ab109041)ab109041 activates AMPK signaling pathway in rat L6 myoblasts.
Differentiated rat L6 myoblasts were stimulated with control buffer or 0.5μg/ml ab109041 for 30min. The cell lysate was subjected to immunoblotting with antibodies against pAMPK and total AMPK.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab109041 has not yet been referenced specifically in any publications.
Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 8.20
Constituents: 0.869% Tris HCl, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute in distilled water
Images
-
Functional Studies - Recombinant human CTRP5 protein (ab109041)ab109041 activates AMPK signaling pathway in rat L6 myoblasts.
Differentiated rat L6 myoblasts were stimulated with control buffer or 0.5μg/ml ab109041 for 30min. The cell lysate was subjected to immunoblotting with antibodies against pAMPK and total AMPK.